Re: SDS Conc. for MALDI

MSweeney (mattsweeney@earthlink.net)
Thu, 17 Jun 1999 17:11:16 -0700

The article from
11th December 1997,=A9 1998 ABRF
STRUCTURAL ANALYSIS OF PROTEINS AND PEPTIDES IN THE PRESENCE OF DETERGENT=
S:
TRICKS OF THE TRADE
by Kristine M. Swiderek, Andrew J. Alpert, Amos Heckendorf, Kerry Nugent =
&
Scott D. Patterson
is at
http://www.abrf.org/ABRFNews/1997/December1997/dec97detergent.html
It is a great source of information in this area.
I did not see the table I think I remember too however.

Matt Sweeney
mattsweeney@earthlink.net
Mass Spec Consulting
Training/Operations/Consulting/Method Development
LC/MS Pharmacokinetics, Peptides, Proteins, Metabolism,
Maintenance Classes, Specialist in Finnigan Equipment and Software

-----Original Message-----
From: James Lee <James_Lee@macmailgw.dfci.harvard.edu>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Date: Thursday, June 17, 1999 7:28 AM
Subject: SDS Conc. for MALDI
>Hi All,
> Sometime in the not so distant past I remember reading an article or
someone
>mentioning something about the critical % of SDS that can be used when
>analyzing samples by MALDI. I seem to remember what was said was that l=
ow
>levels(<0.01%) of SDS were fine and surprisingly levels above 1% or 2% w=
ere
>fine. Does anyone recall reading about this or am I just having some
wishful
>thinking. Thanks.
>
>Jim Lee
>MBCF at Dana Farber Cancer Institute
>44 Binney Street
>Boston, MA 02115