gene synthesis

Shalom David Goldberg (sdg11@columbia.edu)
Sun, 20 Jun 1999 00:04:41 -0400 (EDT)

Hi All,

I need to synthesize a gene that is about 100 bp long. I'm planning to
use a PCR-based method, using overlapping oligos. I would really like to
know if there's anyone who has done a PCR-based gene synthesis, especially
of a long gene, how well it worked, and suggestions for construction of
the oligos (ie length of overlap, oligo size, etc.)

Thanks,
Shalom Goldberg