gene synthesis

Shalom David Goldberg (sdg11@columbia.edu)
Sun, 20 Jun 1999 13:48:53 -0400 (EDT)

The gene is actually around 1100 bp long - I mistyped - this is the
difficulty.

Shalom Goldberg

---------- Forwarded message ----------
Date: Sun, 20 Jun 1999 00:04:41 -0400 (EDT)
From: Shalom David Goldberg <sdg11@columbia.edu>
To: abrf@aecom.yu.edu
Subject: gene synthesis

Hi All,

I need to synthesize a gene that is about 100 bp long. I'm planning to
use a PCR-based method, using overlapping oligos. I would really like to
know if there's anyone who has done a PCR-based gene synthesis, especially
of a long gene, how well it worked, and suggestions for construction of
the oligos (ie length of overlap, oligo size, etc.)

Thanks,
Shalom Goldberg