There are no simple answers as it depends on the purity of your protein
and the characteristics of your protein. I am sure others will give you =
a
good list of things to try. However, of the many approaches, I find the u=
se
of 2M urea in a buffer the most effective and reversible.=20
Hope this helps!
Tony
At 07:27 PM 6/23/99 +0200, Maria Lorenzi wrote:
>Hi, I am Maria Lorenzi.
>I would like to know if there are particular methods to solubilize a pro=
tein
>without loosing its activity.
>Thanks
>Maria
>
>
>
>
>
>
>
>
>
>
>
>
>
>"Soltanto una cosa =E8 veramente importante da fare; ho cercato ma
>non sono riuscita a fartela vedere. So comunque che un giorno la vedrai
>perch=E9, come il sole, =E8 per tutti"
>=20
> M.L.
>************************************************************************=
*
>Dott.ssa Maria Lorenzi
>Dottoranda in Biochimica e Biofisica
>Istituto di Biochimica
>Facolt=E0 di Medicina e Chirurgia
>Facolt=E0 di Agraria
>Universit=E0 degli Studi di Ancona
>Via Ranieri 60131 Ancona, Italia
> Tel(lab):+39 71 220 4395
> +39 71 220 4681
> +39 71 220 4677
> Fax: +39 71 280 2117
> e-mail: mlorenzi@ascu.unian.i=
t
>************************************************************************=
**
>
************************************
Dr. Anthony T. Yeung, Ph.D.
Director, Fannie E. Rippel Biotechnology Facility
Member, Institute for Cancer Research
Fox Chase Cancer Center
7701 Burholme Ave. Philadelphia, PA 19111
Voice: 215-728-2488
FAX: 215-728-3647
email: AT_Yeung@FCCC.edu
http://www.fccc.edu/research/labs/yeung/
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