Thank you so much to those who responded to my question about peptide
cyclization methods. Your suggestions were wonderful;I now have a much better
idea of what is involved in cyclization.
However, I have run into a new dilemma. I am trying to cyclize pentaGly. I have
synthesized and cleaved the peptide. Unfortunately, PentaGly is not at all
soluble in DMF or any other organic solvent that I have tried (I've tried DCM,
NMP, DMF with TFE or DIEA). In fact, the only sovent that I have found that
will dissolve the peptide is rather concentrated aqueous acetic acid.
Does anyone have any suggestions for cyclizing such an uncooperative peptide?
Thank you so much.
Kathy Schegg
Department of Biochemistry
University of Nevada, Reno
Reno, NV 89557
(775) 784-4058
schegg@unr.edu