There shouldn't be any problem with most FPLC resins although some size exclusion matrices may yield voids in the packed bed due to the temperature change. To get around this just hook the column up to the pump as normal and start flowing at the suggested flow rate BUT USING ROOM TEMPERATURE BUFFER in the reservoirs of your FPLC system at the start. The buffers will cool to the cold room temp over a couple of hours and thus gently bring the column to the cold room temperature. Carefully check for column inlet voids after 10-20 column volumes of buffer have gone through the column and top off as necessary.
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Gordon Alton, PhD
Gene and Protein Discovery Group
Signal Pharmaceuticals Inc.
5555 Oberlin Drive
San Diego, CA 92121
Email: galton@signalpharm.com
Phone: 619-558-7500 x8252
Fax: 619-623-0870
WWW: http://www.signalpharm.com
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-----Original Message-----
From: Aki Hoji [SMTP:akhst7@imap.pitt.edu]
Sent: Tuesday, June 29, 1999 8:01 AM
To: Recipients of ABRF List
Subject: FPLC Column and temperature
Dear All,
I packed a FPLC column at the room temperature, and was told that I
can't use the column in a cold room since bubles would form. If this is
ture, is there anyway that I can get away with this problem ? Thanks in
advance.
Aki Hoji
Dept. Infectious Diseases & Microbiology
University of Pittsburgh