I am not an expert on the specifics of FPLC, but the general
chromatographic issues are these:
1. Gases are less soluble as temperature is increased. Therefore, you are
more likely to get bubbles from a column poured in the cold and brought to
room temperature than vice verse.
2. All columns will expand or contract with temperature.
It is generally the case that columns are packed at the temperature at
which they are used (presumably because of point 2). See, for example, p.
179 in "The Tools of Biochemistry" by T.G. Cooper. Some guides stress that
the area in which a column is poured should be free of drafts, to minimize
abnormalities in packing.
Hope this helps.
Chris Halkides
>Dear All,
>
>I packed a FPLC column at the room temperature, and was told that I
>can't use the column in a cold room since bubles would form. If this is
>ture, is there anyway that I can get away with this problem ? Thanks in
>advance.
>
>Aki Hoji
>Dept. Infectious Diseases & Microbiology
>University of Pittsburgh
Christopher Halkides
Dept. of Chemistry, UNCW
601 S. College Road
Wilmington, NC 28403-3297
(910) 962-7427