With open chromatographies like Sephadex, one way to get rid of bubbles
that form at room temp is to put the column in the cold. Or, if you
discover bubbles in a cold column, de-gas some buffer at room temp (where
gasses are anyway less soluble) and then run the degassed buffer thru the
column in the cold. If you have an enzyme that is oxygen-sensitive, degas
at room temp and sparge with nitrogen in the cold using a "gas dispersion
tube" (available in the Chem Dept Stockroom).
Another way to look at it - this is why you need to keep Coke, beer and
champagne cold once opened..
Regards,
John
At 10:00 AM 6/29/99 -0500, you wrote:
>Dear All,
>
>I packed a FPLC column at the room temperature, and was told that I
>can't use the column in a cold room since bubles would form. If this is
>ture, is there anyway that I can get away with this problem ? Thanks in
>advance.
>
>Aki Hoji
>Dept. Infectious Diseases & Microbiology
>University of Pittsburgh
>
>
John Hempel, PhD Ph (412) 624 0161
University of Pittsburgh FAX (412) 624 4759
Department of Biological Sciences
Clapp Hall 301
Pittsburgh PA 15260 USA
email: hempel@psc.edu
http://www.pitt.edu/~biology/faculty/hempel.html