This is an old trick that was published by Angelika Goerg a while ago
(1997, in Electrophoresis, if I am not mistaken).
Using the multiphor, I simply have the electrode contact pad in 10 mM DTT
(in water).
Axel Ducret (in vaccation, but I cannot resist to look at my e-mail.
On Tue, 29 Jun 1999, Katheryn Resing wrote:
> A while ago, someone mentioned putting a filter paper strip with 20 mM DTT
> on IEF cathodes to minimize streaking, but I have lost that e-mail. So I
> am resorting to sending this question to everyone, and apologize in
> advance.
>
> Do you mean putting an extra piece of filter paper above the electrode, on
> the gel portion of the strip, or underneath the electrode, so that they are
> on the plastic portion of the strip? Or do you just add the 20 mM DTT to
> the electrode contact pad itself?
>
> Thanks in advance for help on this
>
> Katheryn Resing
>
>