Re: Protein purification
chris halkides (halkidesc@UNCWIL.EDU)
Thu, 08 Jul 1999 19:55:24 -0400
>Date: Thu, 8 Jul 1999 15:31:11 -0400
>To: Geoff Dwyer <dwyer@central.murdoch.edu.au>
>From: chris halkides <halkidesc@uncwil.edu>
>Subject: Re: Protein purification
>Cc:
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>Hello Geoff,
>
> In talking occasionally with folks who do fungal work, I suspect
>that the protein purification contaminants there are a bit different from
>bacterial sources. Some are easily oxidized. I don't know what to do
>about the specific problem that you indicate, but I would probably seek
>out specialists at places like Dow-Elanco, which have alot of interest in
>fungal enzymes.
> Plant tissues contain phenolic compounds (Suelter, "A Practical
>Guide to Enzymology," Wiley, 1985, p.71) that can be removed with 2%
>insoluble polyvinylpyrrolione. See also Scopes ("Protein Purification,
>Springer), p. 29.
>
> Hope this helps.
>
>Chris
>
>
>
>
>
>>Dear ABRFers,
>>
>>probably a simple question for the protein buffs.
>>
>>I have a student that has precipitated a crude fungal protein extract using
>>acetone but finds that other gunk, most probably carbohydrate materials,
>>have co-precipitated at the same time. Can anyone recommend a simple (or
>>more detailed) method for purifying proteins from either/or fungal,
>>bacterial or plant extracts, that would circumvent co-precipitation of
>>other compounds. We are relatively new to the protein world and would
>>therefore appreciate any feedback we could get for simple protein
>>purification methods from all organisms. Relevent references would also be
>>appreciated.
>>
>>Cheers,
>>
>>Geoff
>>
>>
>>****************************************************************************
>>Geoffrey Dwyer
>>WA State Agricultural Biotechnology Centre,
>>Division of Science and Engineering,
>>Murdoch University,
>>6150 Perth, Western Australia
>>
>>Ph: +61-8-9360 2598
>>Fax: +61-8-9360 6303
>>http://wwwscience.murdoch.edu.au/centres/sabc/
>
Christopher Halkides
Dept. of Chemistry, UNCW
601 S. College Road
Wilmington, NC 28403-3297
(910) 962-7427