Unfortunately, when CNBr cleaves at Met the Sulfur is lost and your
peptides will no longer be labelled!
Ken
Kenneth A. Walsh
E.W.Davie/ZymoGenetics Chair of Biochemistry
Box # 357350
University of Washington
Seattle WA 98195
walsh@u.washington.edu
Phone 206-543-1768
FAX 206-685-9231
On Fri, 9 Jul 1999, Kathryn Sunn wrote:
> Dear All
>
> I am wanting to do some CNBr digestions on a S35 Met labelled
> immunoprecipitated protein, eluted from a gel slice. The peptide of
> interest is approximately 1.8kDa. Does anyone have any ideas as to what
> percentage SDS-PAGE gel I should run these labelled peptides on, and if
> special running buffers (and or gel component buffers) are required?
>
> Thanks in advance
>
> Kate
>
> ___________________________________________________________________
> Kathryn Sunn BSc (Hons)
> Bone and Mineral Research Program
> Garvan Institute of Medical Research
> St Vincent's Hospital Tel: (02) 295 8260
> Sydney NSW 2010 Fax: (02) 295 8241
> Australia email: k.sunn@garvan.unsw.edu.au
> ___________________________________________________________________
>
>
>
>