A 2.1 mM diameter (a typical Brownlee/PE Biosystems column)x 3 cm
radius = .105 "cm" Squared = .01103 Times Pi = .03464
times 3 "cm" = .1039 cm (cubed) or 103.9 microliter volume. A fairly "big"
column.
A smaller column like Vydac sells 1 mm x 50 mm would easily fit these
requirements as it's around 39.27 ul volume. A flow rate of 50 ul/minute is
easily handled with this column. No special frits needed. You should
switch over to .005" ID tubing though if you want to see much of a signal.
If you really want to get small then you'll have to check with our Microm
and LC Packings friends for prepacked small columns. You can always pack
your own "tubing" using the PerSeptive/PE Biosystems Cap. Perfusion tool kit
(P/N 5-2218-00) and that will give you column volumes in the .6 to 10 ul/5
cm range.
-----Original Message-----
From: lisa bibbs [mailto:bibbs@scripps.edu]
Sent: Tuesday, July 20, 1999 11:39 PM
To: Recipients of ABRF List
Subject: Fwd: Got microcolumns?
Can any of you help me with this one?
>X-Sender: mathison@gcrc.scripps.edu
>Date: Tue, 20 Jul 1999 07:11:33 -0700
>To: bibbs@scripps.edu
>From: John Mathison <mathison@scripps.edu>
>Subject: Got microcolumns?
>Mime-Version: 1.0
>
>7/20/99
>
>Hi Lisa,
>
> I need to do some microchromatography.... i.e. bed sizes of 50-100
>ul or
>so. If the minimum accepable bed height were 2 or 3 mm, then the column
>diameter would need to be on the order of 2.5 mm (no matter how you slice
>the pi). I can imagine such a beast with witty bitty flow adapters and
>frits, but where could you buy it?
>
>John Mathison
>4-8547
>