Re: 96-well post-sequencing cleanup devices

Robert Lyons (boblyons@umich.edu)
Wed, 21 Jul 1999 20:46:26 -0400

Tom Howard wrote:
>
> Does anybody know of a company which makes a 96 well microtiter dish based
> device which can do cleanup (removal of the unincorporated difluorophor
> terminators) of post-cycle sequencing reactions prior to gel loading? If
> such devices exist, is there a speedvac with rotors capable of spinning
> these microtiter devices?

Princeton Separation (Princeton, NJ) makes one. They are reliable, and
well-proven, but a bit on the expensive side (about $1 per well in modest
quantities, I think $0.80/well in larger quantities). Millipore makes a
pack-your-own version that as I recall comes in at $0.30 per well. A few
other companies make such products as well - perhaps others can fill you
in on those.

We concentrate ours in a Savant SC200 SpeedVac with a rotor designed for
microtiter plates. We had to modify the buckets to accommodate the double-
decker plate+manifold combination (the buckets were intended to hold two
standard 96-well plates in an over/under config; we removed the middle
platform so the remaining space could hold a taller plate assembly).

BTW, the 'abrf-request' address is not for posts. It's only for subscribe
and unsubscribe requests.

Bob Lyons
University of Michigan