You may run the peptide through a gelfltration column (Sephadex G-25
superfine) using dilute HCl or stir the peptide with an anion exchange
resin.
Henriette
>ABRFers,
>
>I need to exchange the counterion on a peptide from TFA to HCL. I
>remember a while back a discussion about how best to do this. Someone
>mentioned that you could just use a reversed-phase column to do this.
>Has anyone tried this? Does anyone have a reliable protocol for this?
>
>Cheers,
>
>=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-
>Gary Van Domselaar gvd@redpoll.pharmacy.ualberta.ca
>Faculty of Pharmacy Phone: (780) 492-4493
>University of Alberta FAX: (780) 492-5305
>Edmonton, Alberta, Canada
Henriette A. Remmer, Ph.D.
Director of Protein Services
Protein Sciences Facility at the Biotechnology Center
University of IIllinois
315/311 Noyes Laboratory, Box 62-1
505 S Mathews Ave Urbana, IL 61801
phone: (217)333-4695 (lab) or (217) 333-3841 (office)
fax: (217)244-1142 email: remmer@uiuc.edu
homepage: http://www.life.uiuc.edu/biotech/protein_sc.html
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