RE: 96-well post-sequencing cleanup devices

Leviten, Dina (dleviten@icos.com)
Thu, 22 Jul 1999 11:29:15 -0700

Tom,
There are several companies: Princeton Separations, EdgeBiosystems, BioRad,
Millipore and Qiagen is coming out with one. I'm sure there are other
companies too. We have tested the first two and Millipore and the best
results came from Princeton. The cheapest is Millipore. Jouan rotors or
Beckman tabletops work, but be sure and specify special plate holders that
will accommodate the entire stack heigth. We use a Savant system to dry
down the plates and it works great.
Hope this helps... Let me know if you want more details.
Dina Leviten
ICOS, Corp
dleviten@icos.com

> -----Original Message-----
> From: Tom Howard [SMTP:thoward@emory.edu]
> Sent: Wednesday, July 21, 1999 2:35 PM
> To: Recipients of ABRF List
> Cc: abrf@aecom.yu.edu; abrf-request@aecom.yu.edu
> Subject: Re: 96-well post-sequencing cleanup devices
>
> Does anybody know of a company which makes a 96 well microtiter dish based
> device which can do cleanup (removal of the unincorporated difluorophor
> terminators) of post-cycle sequencing reactions prior to gel loading? If
> such devices exist, is there a speedvac with rotors capable of spinning
> these microtiter devices?
>
> Tom E. Howard
> Assistant Professor
> Department of Pathology
> Emory University School of Medicine
> 1639 Pierce Dr.
> Atlanta, GA 30322-3050
> Ph. 404-727-3729
> Fax. 404-727-3404
> E-mail. thoward@emory.edu
> Pager: 404-686-5500 (PIC#: 10442)