Re: ESI-MS in negative ion mode

MSweeney (mattsweeney@earthlink.net)
Thu, 22 Jul 1999 18:47:03 -0700

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I saw some work using ammonium at +7 pH in combination with a PLRP C-18 =
RP column (too avoid silica dissolution) for decent LC/MS on =
phosphopeptides using ESI in the negative ion mode.

Matt Sweeney
mattsweeney@earthlink.net
Mass Spec Consulting
Training/Operations/Consulting/Method Development
LC/MS Pharmacokinetics, Peptides, Proteins, Metabolism,
Maintenance Classes, Specialist in Finnigan Equipment and Software

-----Original Message-----
From: Dougie Lamont <dougie01@globalnet.co.uk>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Date: Wednesday, July 21, 1999 7:50 PM
Subject: ESI-MS in negative ion mode
=20
=20
Hi all,
=20
I'm interested in analysing acidic peptides in negative ion mode. =
Has anyone done this type of work and could provide some information on =
the best method of analysis (online-LCMS or infusion),buffers etc.=20
=20
Thanks=20
=20
=20
Dougie Lamont
=20
PPL Therapeutics

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I saw some work using = ammonium at=20 +7 pH in combination with a PLRP C-18 RP column (too avoid silica = dissolution)=20 for decent LC/MS on phosphopeptides using ESI in the negative ion=20 mode.
 
Matt = Sweeney
mattsweeney@earthlink.netMass=20 Spec Consulting
Training/Operations/Consulting/Method = Development
LC/MS=20 Pharmacokinetics, Peptides, Proteins, Metabolism,
Maintenance = Classes,=20 Specialist in Finnigan Equipment and Software
 
 
-----Original = Message-----
From:=20 Dougie Lamont <dougie01@globalnet.co.uk>=
To:=20 Recipients of ABRF List <abrf@aecom.yu.edu>
Date:=20 Wednesday, July 21, 1999 7:50 PM
Subject: ESI-MS in = negative=20 ion mode

Hi all,
 
I'm interested in analysing = acidic peptides=20 in negative ion mode. Has anyone done this type of work and could = provide=20 some information on the best method of analysis (online-LCMS or=20 infusion),buffers etc.
 
Thanks
 
 
Dougie Lamont
 
PPL=20 Therapeutics
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