Re: DNASeq Internal Standards

Robert Lyons (boblyons@umich.edu)
Fri, 23 Jul 1999 15:42:23 -0400

James Farmar wrote:
>
> We are looking for a source of pcr products as internal controls for our dna
> sequencing.
>.... we could avoid the usual back-and-forth between researcher and
> sequencer about the cause of a failure to obtain sequence from a sample.

My honest opinion is that you would gain little from such a control.
It is a fact of life that, when an experiment fails, the researcher
will look for someone to blame it on. Core facilities are too easy
a target for blame.

We all know what research is like: you work day and night, setting
up a complicated experiment - and it fails. You're SURE the sample
was fine, the primer worked before, the experimental design was
impeccable. "I'll bet that damn Core screwed up."

You'll show them a standard that proves you can sequence a PCR
product, but it's not their primer and not their DNA. They'll
frankly still be suspicious that your technician screwed up.

I know of no commercially-available PCR products. best I can suggest
is that you simply keep on hand a few successful PCR reactions to
demonstrate your prowess. Good luck!

Bob Lyons
University of Michigan