Re: RE: 18O-enriched water

Elliott Nickbarg (ENickbarg@genetics.com)
Fri, 23 Jul 1999 16:29:50 -0400

When this question arose a while ago, I reported that one could obtain O-18 water from Oak Ridge Nat. Lab. That is no longer true. They now have only isotope-depleted O-16 in their catalog (are they throwing away the O-18?). However, Novachem lists it in their catalog. Check out the following:
http://www.novachem.com.au/products.htm

Elliott Nickbarg
Genetics Inst. Inc.
>>> "LANGE, GARY W [PHR/1005]" <GARY.W.LANGE@stl.Monsanto.com> 07/23 9:36 AM >>>
I obtained water from Aldrich in April of this year cat# 32,987-8. It's a
neat little trick for tryptic digests. If you are counting disulfide bonds
too those tryptic fragments will have a +4 if they don't contain the
C-terminus. There also was a little paper on using O18 technique for
assigning Asn linked carbohydrates. I think however I would suggest
performing the PNGase F treatment after tryptic digestion of the gel slice
instead of before digestion as it works much faster on smaller peptides.

Gary Lange
g.w.lange@monsanto.com

-----Original Message-----
From: Wu,Jiang [mailto:Jiang.Wu@add.ssw.abbott.com]
Sent: Thursday, July 22, 1999 4:04 PM
To: Recipients of ABRF List
Subject: 18O-enriched water

Does anyone know where I can buy 18O-enriched water in the country. I was
told Cambridge Isotope Lab discontinued to supply. Thank you very much.

Jiang

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