Antipeptide antibodies

Latif Kazim (kazim@sc3101.med.buffalo.edu)
Tue, 27 Jul 1999 13:19:35 -0400

Dear Colleagues:

One of our researchers recently asked the following question about a method
for making antipeptide antisera:

>Do you have knowledge/experience with direct use of synthetic
>peptides for producing antisera--particularly the 6xhis-tagged peptide
>method?? Apparently the peptide is bound to Ni-NTA agarose and used
>directly.

Any responses would be appreciated.

Thanks,

Latif Kazim

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A. Latif Kazim, Ph.D.
Biopolymer Facility
Science-4
Roswell Park Cancer Institute
Elm & Carlton Sts.
Buffalo, NY 14263

E-mail: kazim@sc3101.med.buffalo.edu

Phone: (716) 845-8923
Fax: (716) 845-7621