RE: Acetylation of a cleaved peptide

Chin, David T. (ChinD@missouri.edu)
Wed, 28 Jul 1999 10:46:22 -0500

Bob,
With the C-terminal lys, it will be easier just to resynthesize the
peptide. Reactivity a-NH2>e-NH2 at pH 8 or below, so you have a change of
acetylation by doing the reaction at pH 7.8.

David Chin

-----Original Message-----
From: Bob Lee [mailto:boblee@uic.edu]
Sent: Tuesday, July 27, 1999 4:35 PM
To: Recipients of ABRF List
Subject: Acetylation of a cleaved peptide

Hi, need suggestion to put a acetyl group at the N-terminus of a cleaved
and purified peptide GHGLGHGHEQQHGLGHGHK. Thanks.

Bob Lee