DNAseq

Margarita Almeida (malmeida@molbio.med.miami.edu)
Wed, 28 Jul 1999 12:51:30 -0400

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I work in a DNA core facility and I am having problems sequencing large
templates (BAC,PAC,COS & P1). Since the samples received are prepped in a
variety of way, I was wondering if anyone out there could suggest any
protocols that I could suggest to my customers. I would also like to know if
anyone has any suggestions for the reaction set up and/or post reaction
purification. I am using an ABI 373a with XL upgrade and the "Big Dye"
Terminator kit. Thanks.

Margarita Almeida

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