PE sells cellulose combs in a 96 lane format (other sizes also avail). You
cast the comb with the standard plastic comb, but when ready to load you
insert the cellulose comb and add your top running buffer. They work best if
you allow them to sit in the buffer for about 5 min before loading samples
to allow it to swell. Also, once you've finished loading your gel, you run
the gel for about 2 min and then remove the comb and toss it.
We've found better defined peaks, better read lengths, easier tracking and
most of all ALMOST NO SAMPLE BLEED!
Give them a call. They come in boxes of 50 combs. Suffice it to say that we
have a stock of 800 on hand...
Chris Jacobs
DNA Sequencing Lab
Parke-Davis Pharmaceuticals
Office Phone: (734) 622-5724
Lab Phone: (734) 622-1815
E-mail: chris.jacobs@wl.com
> ----------
> From: Tom Howard
> Reply To: Tom Howard
> Sent: Wednesday, July 28, 1999 4:06 PM
> To: Recipients of ABRF List
> Subject: PE ABI 377 Sequencer
>
> Thanks for all of your responses and suggestions regarding my question
> about
> post-sequencing product cleanup in a 96 well plate format. Yesterday I
> loaded my first 96 well sequencing gel for the ABI-377 using the Kloehn 8
> Channel syringe gel loader recommended by PE. We had some difficulties
> with
> movement of the comb during insertion and removal of the needles at sample
> loading. I know that this caused sample leakage into adjacent wells. But
> what I didn't expect was that all of the DNA sequences (from every lane)
> ran
> diagonally to the left (if facing gel). The left side of the comb
> corresponded to the side that I saw the most leakage. Could a slightly
> malpositioned comb cause this deviation of the runs from vertical? If so,
> how can I keep the comb tightly in place at loading so that the snuggly
> fitting needles don't continue to shift it. Can we tape it at top or
> maybe
> clamp it in some way? Any suggestions will be much appreciated.
>
> Tom E. Howard
> Assistant Professor
> Department of Pathology
> Emory University School of Medicine
> 1639 Pierce Dr.
> Atlanta, GA 30322-3050
> Ph. 404-727-3729
> Fax. 404-727-3404
> E-mail. thoward@emory.edu
> Pager: 404-686-5500 (PIC#: 10442)
>