RE: cysteine alkylation

Chin, David T. (ChinD@missouri.edu)
Thu, 29 Jul 1999 14:56:12 -0500

Lyuben,
Urea and GnHCl are used because they are ready available in pure form
(i.e., inexpensive) and easy to remove by dialysis or size exclusion
columns. SDS is common, but difficult to remove. However,SDS/urea solution
are commonly used in 2D gels for the second-D sample buffer ( for the
equilibration and alkylation steps). Note that GnHCl and SDS are not
compatible.

David T. Chin
Director, Protein Core Facility
Protein Chemistry and Expression
2-17 Agriculture Building
Univ. of Missouri - Columbia
Columbia, MO 65211-7170

SHIPPING AND ACTUAL LOCATION:
2-17 Agriculture Building (office)
2-31 Agriculture Building (Lab)

e-Mail: chind@missouri.edu
web: http://www.biotech.missouri.edu/pc
[mailto:chind@missouri.edu]
Phone: 573-882-2027
Fax: 573-882-7105

-----Original Message-----
From: Lyuben Marekov [mailto:lmarekov@Box-l.nih.gov]
Sent: Thursday, July 29, 1999 12:14 PM
To: Recipients of ABRF List
Subject: cysteine alkylation

Hello everyone,
While urea and GnHcl are universally used for solvents/denaturants for
proteins has anybody ever attempted to do cys alkylation in SDS solutions.

Lyuben

**********************************************************************
Lyuben N. Marekov phone: (301)-496-7190
NIH, NIAMS fax: (301)-402-2886
Bldg 6/Room 128 e-mail: lmarekov@box-l.nih.gov
Bethesda, MD 20892-2752
**********************************************************************