I've recently posted a note on cloning kits, but maybe my problem is
preparation for ligation from my Vent amplified PCR product. My primers
are designed with restriction sites. After PCR, I purify my product and
do a restriction digest on my PCR product to produce sticky ends. Should
I be doing this or go for a direct ligation?
Thanking everyone in advance.
Sue
Samuel Lunenfeld Research Institute
sroka@mshri.on.ca