I use a cocktail of 90%TFA, 8.5% Water, and 1.5% TIS for a 34-mer with 3
Arg's in a row with out any noticible Pbf peaks by mass spec. I also found
that this seems to suppress linker cleavage as well...+163 w/o the Nle spacer
and +275 with the spacer on MBHA amide resin.
Best part is...no smellies!!! But, if you have any Met or Cys in your
peptide then they are a necessary evil.
Regards,
Steven Johnson
Research Associate, Biomeasure, Inc.