Can you please clarify what you mean by the 'ABRF one'? I don't recall the
ABRF producing an endorsed method. Do you mean the method presented by
Kathy Stone at ABRF96 tutorial from the Keck Facility or to methods
suggested in the literature which has accompanied test samples from ABRF?
I'm sorry if this seems a bit picky but I don't want the ABRF to take
credit for methods which have been developed by others. If a method has
been developed by an ABRF Research Group, then that is a different matter.
On a separate issue, thanks to those who have given protocols for AspN
digestion. Can I ask contributors in general to avoid using greek
characters in messages because it is clear that some values which are
intended as microlitres (ul) are appearing as millilitres (ml), presumably
because 'mu' on some keyboards is alt-m and the formatting is not
recognised by some mailers. Please stick to 'u' for 'mu'. Thanks.
Len
>I used the same protocol as for trypsin (the ABRF one), but make sure you
>digest a blank spot because I got a lot of asp-N peptides. You might want
>to try a test set of digests, varying asp-N, to determine the minimum
>amount of asp-N you need, using myoglobin at about the same protein
>concentration as your sample.
>
>Katheryn Resing
*********************************************************************
Dr Len C. Packman
Assistant Director of Research
Protein and Nucleic Acid Chemistry Facility
Department of Biochemistry
University of Cambridge
80 Tennis Court Road
Old Addenbrookes Site
Cambridge, CB2 1GA, UK
Tel: +44 (1223) 333639
FAX: +44 (1223) 766002
e-mail: lcp2@mole.bio.cam.ac.uk
Visit my WWW page at http://www.bio.cam.ac.uk/proj/adr/PNAC/pnac.html