Dear ABRFers:
After seeing the post on the anti-peptide antibodies, I was
wondering if anyone could give me feedback on our experience with
making these. We made 12 mer peptides from 85 different proteins
(selected for hydrophilicity, antigenicity, etc using DNASTAR
program). Coupled to BSA via the terminal cysteine that we added
using SMCC, then injected into rabbits. The resulting antibodies
were purified on peptide columns. Only about 20% of the
antibodies reacted with the respective proteins in Western blots
under reducing conditions. Any idea whether this is unusual?
Thanks very much.
Dick Wicks
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