Re: MS source

Tim Slattery (Tim_Slattery@berlex.com)
Tue, 03 Aug 1999 9:52 -0800

Joe, I have the Protana adapter for my LCQ. I really can't compare it with the
New Objective device since I've never use one. However, I one thing I did learn;
if you get the PROTANA, don't get their camera and monitor system. It uses
European voltages (they give you a transformer to adapt to US voltages) and CCIR
format which is not common in this country. If you wish to capture images on
computer or use these cameras for any other purpose you are putting yourself at
a disadvantage. I would recommend buying cameras and monitors from someplace
like Edmund Scientific (www.edsci.com).

In this lab we analyze protein/peptides samples and small molecule organics, so
I can't comment of sugars and lipids, however, mixing low level samples and high
level samples not a good idea if you can avoid it. An overloaded sample can take
a long time to clean out of the LCQ. Frequent loading of concentrated samples
also will require more frequent disassembly of the Stack for cleaning to keep
background noise down. If I had a choice I'd avoid trying to mix high
sensitivity MS/MS and general use.

Good Luck
--tks
Tim Slattery
Protein Biochemistry and Biophysics Department
Berlex Biosciences
tim_slattery@berlex.com

______________________________ Reply Separator _________________________________
Subject: MS source
Author: msssf@pilot.msu.edu (Joe Leykam) at Internet
Date: 8/2/99 9:50 AM

Greetings All

I have two questions for the MS experts.

We will receive a LCQ deca in the next few weeks and I am unsure of which
flow adaptor to purchase:
The PROTANA or New Objective. I am interested in the pro and cons of each
nano srpay source.

The second question is about mixing samples. The primary use will be for
protein sequencing, but we have several users who wish to use the LCQ for
sugars and lipids. Any comments would be welcome
on mixing high sensitivity MS/MS sequencing samples and general MS samples
on the same MS.

Joe Leykam

Joseph F. Leykam Phone (517)353-4824
Director. MSSSF FAX (517)353-9334
Dept. of Biochemistry RM 223
Michigan State University E-mail msssf@msu.edu
East Lansing MI 48824