The following book has a section reviewing methods for TLC/FAB-MS.
No peptides but the methodology would be the same as for the antibiotics,
sugars and pigments it discusses. Never tried it myself. They do a nice
looking TLC band concentration step prior to FAB by scraping the plate
around the spot into a trapezoid and using a syringe of solvent to re-
focus the band into the small end of the trapezoid.
I have scrapped and eluted other compounds from TLC plates. I used
a glass fritted funnel hooked up to a vacuum line with a trap for my
solvent/analyte. I dumped the scrapped silica into the funnel, turned
on the vacuum and washed the silica with some spatula stirring.
Then I concentrated the solvent.
"Chromatography and Mass Spectrometry for Analysis of Natural Products"
Ken-ichi Harada, Makoto Suzuki, Hisao Oka
pages 605-630 in Biological Mass Spectrometry: Present and Future
Ed. T. Matsuo, RM Caprioli, ML Gross, Y Seyama 1994 John Wiley and Sons
In this paper they cite
Nakagawa and Iwatani, 1991 jchrom 562, 99-110
Chai et al., 1991 Biol Mass Spec 20, 169-178
Dunphy and Busch, 1990 Talanta 37, 471-480
Matt Sweeney
mattsweeney@earthlink.net
Mass Spec Consulting
Training/Operations/Consulting/Method Development
LC/MS Pharmacokinetics, Peptides, Proteins, Metabolism,
Maintenance Classes, Specialist in Finnigan Equipment and Software
-----Original Message-----
From: Ejvind Moertz <moertz@protana.com>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Date: Monday, August 09, 1999 2:53 AM
Subject: MS of TLC peptides?
>Does anyone have experience or references to MS analysis of peptides eluted
>from TLC plates?
>
>Any help will be appreciated.
>
>Thank you.
>