Any comments from anyone on the stability of an FITC-peptide conjugate at
low pH (say ~ 2)? I've labelled a decapeptide at the N-terminus with FITC
(i.e. as the isothiocyanate) but it looks as if it's fallen apart on
purification under standard RP-HPLC conditions (i.e. 0.1% TFA). We're
going to look at HPLC at pH 6.5 and at using succinimide ester derivatives
as well, but just wondered if anybody else has had a similar experience
with FITC and peptides.
Cheers,
Roger
------------------------------------------------------------
Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Studley Road,
Heidelberg, Vic. 3084
Australia.
Tel 61-3-94965463
Fax 61-3-94965436
Email Roger.Murphy@Ludwig.edu.au