dna seq

Thomas J. Stelick (tjs11@cornell.edu)
Wed, 11 Aug 1999 13:03:45 -0400

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We are trying to sequence a cloned PCR product for a customer but we are
having a problems. The pcr product has a 24 base inverted repeat at the
ends which is causing to fold on itself and the sequence can not get
through this structure. We have tried DMSO but no difference is seen.
Does anyone have any suggestions on how to get through it.

Tom Stelick
TJS11@cornell.edu
BioResource Center, Cornell University
157 biotech bldg.
Ithaca, NY 14853
(607)254-4857
http://brcweb.bio.cornell.edu

Next message: Bob Lee: "Tritium [H3] labeled peptide"
Previous message: Dougie Lamont: "Difference between API 3 and API100b (Sciex)"
Next in thread: Wagner Yotov: "RE: dna seq"