Protein purification
Matteo Villain (villain@uab.edu)
Wed, 11 Aug 1999 17:06:37 -0500
A friend is looking for a a facility or company willing do do a contract
job.
He has two recombinant protein (no tag, and in inclusion body) that is
express at about 3% of the total protein content. The proteins form a
complex that need denaturation to be disrupted. My guess is the job would be
to separate the complex from the bulk of the protein and then by gel
exclusion in denaturating conditions separate the two sub unit. They have
grams of the crude material.
Anyone interested?
Let me know
Matteo Villain E-mail : villain@uab.edu
Research Associate Phone : 205 934 3032
University of Alabama Fax : 205 934 1446
Birmingham
35294, USA