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Hey Dougie,
I run an "old" API3 and a younger API100 in my lab. The instruments are
quite different, not only that the API3 is a triple quad and the 100 a single
quad we notice that the sensitivity of the 3 is much better. Furthermore the
ion-source possibilites with the 3 are much better, we run nanospray,
microspray (both home-made), normal ion spray, LC/MS, MS/MS, sometimes also
LC/MS/MS. But the instrument is limited on one side, you have to recycle at least
after two days use, better every night to get the condensate out. So long
term analysis with many consequetive LC/MS runs are not possible. We also
notice that the day-to-day mood of our 3 is much changing. This affects
sensitivity, resloution, and also the calibration. As we work in germany we may
start the day with warm climate and low huminity but change to lower
temperature and increased huminity, both sensitivity and resolution are much affected
(we donot have a air condition in the lab). The 100 is more stable, as we
still calibrate every day (which may not be neccessary), we donot see much
changes.
But the 3 is our working horse for all high sensitivity analysis and the
100 we only use for synthetic peptides and LC/MS runs for high concentrated
samples and for pharamaceuticals (sometimes, it is not our main buisiness).
To adapt nanospray or microspray to the 100 further changes have to be done,
because you cannot control all voltage setting independly like with the 3.
I think the instruments are different from their personality, the 3 often
changes its moods and has to be treated carefully and polite, while the 100
never shows any moods, except that with dirty samples the orifice gets very
fast closed and it has to be cleaned. To clean the orifice is some work at
the 3 it takes at least 2 - 3 hours and an overnight recycling while the 100
is ready within 1 - 2 hours from switching off and opening.
As you can see it is not easy to explain the differences in a short mail,
at least both instruments are no longer build and replaced by newer and even
more sensitive mass specs. But as I will leave my 3 and 100 soon, I will
miss the instruments very much.
Hope this answers some of the questions, please feel free to contact me
any time.
Yours
Manfred
Manfred Raida
IPF (until end of september)
Feodor-Lynen Strasse 31
D-30625 Hannover
Germany
Tel +49 511 5466 123
Fax +49 511 5644 132
email mraida@gmx.de
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