There are cheap ways and expensive ways to do this. None need involve
putting radioactivity through your synthesizer. The inexpensive route will
work if the peptide can be made with a blocked amino terminus. At the end
of the synthesis, remove the Fmoc group, then in the hood acetylate the
amino terminus with hot acetic anhydride. Depending on whether (and how
much) cold compound you use, you can control the specific activity of the
peptide. Alternatively, incorporate into your synthesis an amino acid such
as dehydroproline, dehydroleucine, or dehydroalanine (I'm assuming that the
..... represented other amino acids). You can send the purified peptide to
NEN or Amersham for catalytic hydrogenation. This will give you very high
specific activity, and restore the natural sequence. As Alfred Chung
suggested, I believe that iodotyrosine will work for this purpose as well.
This latter method is expensive, and perhaps more suited for long term
projects. If your peptide has a cysteine, and it can be modified, another
inexpensive way is simply to modify it with tritiated iodoacetic acid or
iodoacetamide.
Good luck,
Ruth
At 02:19 PM 8/11/99 -0400, Bob Lee wrote:
>Hi everyone,
>
>Need suggestion on how to make tritium [H3] labeled WKYMV.....
>Thanks.
>
>
>Bob
>
>
>