Re: Tritium [H3] labeled peptide
David A. Schooley (schooley@med.unr.edu)
Thu, 12 Aug 1999 10:37:54 -0700
Bob-
I don't quite understand the question. As stated earlier, In my
opionion you need to substitute Nle for Met. If this is OK, and you did
not say if it is, then you can treat the peptide with any of a number of
reagents designed for radioiodination of peptides, except using non-labeled
iodine. You will probably get a mix of mono- and di-iodo tyr species. You
may easily separate the two by HPLC. Then ship it to a company such as
NEN, and they will conduct a catalyic tritiolysis with Pt or Pd catalyst,
replacing each iodine (if you have two) with a triton. They will remove
labile tritum as part of the service, but purification of the product
generally costs more. The monoiodo species will have better long term
stability, but of course lower specific activty
David
Dear David,
Can you give me some references and details (companies) about how to do this?
This is our first time doing this.
Thank you very much.
Bob
Bob-
That is a tough one- the Met poisons Pt or Pd catalysts usually
used for reducing something like O-iodoTyr. Learned that the hard way
within the last year- disulfides and Met make life miserable for tritium
labeling. Can you substitute Nle for Met?
>Need suggestion on how to make tritium [H3] labeled WKYMV.....
>Thanks.
>
>
David A. Schooley
Dept. of Biochemistry/330
Univ. of Nevada
Reno, NV 89557
schooley@unr.edu
tel: (775) 784-4136; fax (775) 784-1419
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