you may try primer T(20)VN (the mixture). For us, this did help in a
number of cases.
Regards, Bernd
>We're running into problem sequencing through the poly T (poly A) area of
>cDNA clones. Too often, we'll see 'stutter'- it looks as though the
>polymerase is slipping on the longer stretches of poly T and all of the
>sequence past that point is ruined.
>
>Has anyone conquered this problem? If so, I'd certainly appreciate some
>pointers.
>
>Thanks-
>Donna Brown
>
>donna@resgen.com
_____________________________________________________________________
PD Dr. Bernd Weisshaar
MPI fuer Zuechtungsforschung
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