I am by no means an expert in peptide synthesis, so forgive me if you find that this question insults your intelligence, but:
It takes alot of DMF -- the protocol I have says 2ml or more /mg. Could it be you haven't gone up in volume enough ?
That's my only idea, if that's not it maybe the experts will have a solution.
-- Rebecca P. Ettling
Biotechnology Resource Laboratory
Protein Sequencing and Peptide Synthesis Facility
Medical University of South Carolina
173 Ashley Avenue, Room 733D BSB
Charleston, SC 29403
Tel.: (843) 792-1271
Fax: (843) 792-1264
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Terry Stoming wrote:
> I have recently made a peptide containing an N-terminal serine. Left the Fmoc on and purified it, including lyophylization. It seems reluctant to go into DMF so I can cleave the Fmoc. Does anyone have any suggestions???
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I am by no means an expert in peptide synthesis, so forgive me if you find
that this question insults your intelligence, but:
It takes alot of DMF -- the protocol I have says 2ml or more /mg. Could it be you haven't gone up in volume enough ?
That's my only idea, if that's not it maybe the experts will have a solution.
-- Rebecca P. Ettling
Biotechnology Resource Laboratory
Protein Sequencing and Peptide Synthesis Facility
Medical University of South Carolina
173 Ashley Avenue, Room 733D BSB
Charleston, SC 29403
Tel.: (843) 792-1271
Fax: (843) 792-1264
---------------------------------------------------
Terry Stoming wrote:
I have recently made a peptide containing an N-terminal serine. Left the Fmoc on and purified it, including lyophylization. It seems reluctant to go into DMF so I can cleave the Fmoc. Does anyone have any suggestions???