Another solvent you can use instead of DMF is N-Methyl-2-Pyrrolidone (NMP).
I have found it useful in synthesis as a better solvent than DMF for some
amino acids/peptides. (It is more expensive.) You can use it with 20%
piperidine to remove your N-terminal Fmoc. Also, try sonicating for 5 min.
Hope this helps,
Jennifer L. Smith
New England Peptide, Inc.
888-343-5974
-----Original Message-----
From: Terry Stoming <TSTOMING@MAIL.MCG.EDU>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Date: Friday, August 13, 1999 9:41 AM
Subject: N terminal serine
>I have recently made a peptide containing an N-terminal serine. Left the
Fmoc on and purified it, including lyophylization. It seems reluctant to go
into DMF so I can cleave the Fmoc. Does anyone have any suggestions???
>
>