You may choose a different organic solvent in which the peptide and
piperidine is soluble. I recently used acetonitrile for a peptide
cyclization in solution. It worked well.
If the peptide is water soluble, try to cleave Fmoc with NaOH.
Unfortunately I don't have a proven protocol or a literature reference.
Henriette
>I have recently made a peptide containing an N-terminal serine. Left the
>Fmoc on and purified it, including lyophylization. It seems reluctant to
>go into DMF so I can cleave the Fmoc. Does anyone have any suggestions???
Henriette A. Remmer, Ph.D.
Director, Protein Sciences Facility
Biotechnology Center
University of IIllinois
315/311 Noyes Laboratory, Box 62-1
505 S Mathews Ave Urbana, IL 61801
phone: (217)333-4695 (lab) or (217) 333-3841 (office)
fax: (217)244-1142 email: remmer@uiuc.edu
homepage: http://www.life.uiuc.edu/biotech/protein_sc.html
*****************************************************