No need to actually dissolve your peptide, in fact it is easier to work up if you don't. I have had excellent results with stirring a cleaved peptide in neat piperidine for about half an hour, (the peptide should stay a solid in the bottom of the flask), decanting the piperidine, washing a few times with ether and drying it completely before re-dissolving it in water to lyophilise. You may need to send it through the prep column again but if you do a good job washing off the piperidine, that should be unnecessary.
good luck,
Jane
Jane A. Nagel MSc
Genetics Institute
Cambridge, MA
1 888 577 1500 x 8597
jnagel@genetics.com
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