Has anyone ever done an immunoprecipitation under non-reducing conditions,
and successfully separated the antigen from the antibody??
My problem is that I need to pre-enrich my protein sample by IP for mass
spec, and have the issue that the heavy chain of the antibody is the same
size as my protein. We dont have the availability of the antibody to couple
it directly to sepharose beads and so need to IP the usual way, and then
separate the protein antigen from the AB-beads without disrupting the
Antibody complex.
Can this be achieved with a high salt buffer??
Thanks in advance
Kate
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Kathryn Sunn BSc (Hons)
Bone and Mineral Research Program
Garvan Institute of Medical Research
St Vincent's Hospital Tel: (02) 295 8260
Sydney NSW 2010 Fax: (02) 295 8241
Australia email: k.sunn@garvan.unsw.edu.au
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