More than likely what you are seeing in the cleavage solution is precipitated
Trt. There maybe some Pbf as well but mostly Trt (of course that assumes you
have Trt protected residues in your sequence). You certainly have enough
volume of reagent ~25uL/mg. I usually notice the precipitate on rotovaping
off the cleavage cocktail prior to workup, but everyones sequences are
different so...
These will solubilize and wash away in your workup...either extractive or
precipitation with ether.
Regards,
Steven Johnson, B.S.
Biomeasure, Inc.
alternate e-mail: labswine@yahoo.com