Re: Cyanogen Bromide cleavage

gsarath@unlnotes.unl.edu
Mon, 16 Aug 1999 09:04:19 -0500

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Dear All: One of my colleagues Dr. Les Lane in the Plant Pathology Department,
told me several years ago that he had used CNI to effect Trp cleavage in
proteins. I tried a few samples with CNI essentially using the CNBr protocols.
There appeared to be some problems with extraneous cutting and the purity of the
CNI we used was not the best. I have never revisited this reagent, but I am
certain that we can readily effect Trp, however, I think with higher pruity
reagents, we should be able to get good and quantitative cleavage at Trp. Maybe
I should try this beasties once again, Gautam

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Previous message: Per-Ingvar Ohlsson: "Deglycosylation on PVDF"
Maybe in reply to: Henry Keutmann: "Cyanogen Bromide cleavage"