RE: Cyanogen Bromide cleavage
Amina S. Woods (awoods@jhmi.edu)
Mon, 16 Aug 1999 13:18:05 -0400
On Monday, August 16, 1999 10:04 AM, gsarath@unlnotes.unl.edu
[SMTP:gsarath@unlnotes.unl.edu] wrote:
> Dear All: One of my colleagues Dr. Les Lane in the Plant Pathology
Department,
> told me several years ago that he had used CNI to effect Trp cleavage in
> proteins. I tried a few samples with CNI essentially using the CNBr
protocols.
> There appeared to be some problems with extraneous cutting and the purity
of the
> CNI we used was not the best. I have never revisited this reagent, but I
am
> certain that we can readily effect Trp, however, I think with higher
pruity
> reagents, we should be able to get good and quantitative cleavage at Trp.
Maybe
> I should try this beasties once again, Gautam
If you want to cleave at Trp you should use N-chloro-succinamide or
N-bromo-succinamide or use BNPS-skatole. Martha Vestling published a very
good paper in Rapid comm on the subject:
Vestling et al. Optimization by Mass Spectrometry of a Tryptophan-Specific
Protein Cleavage Reaction, Rapid Communications in Mass Spectrometry, Vol.
8, 786-790 (1994).
Amina
Amina S. Woods Ph.D.
Johns Hopkins School of Medicine
725 N Wolfe St. Baltimore, MD 21205
Tel: (410) 614-4981, Fax: (410) 955-3420
E-mail: awoods@jhmi.edu