Liz Higgins
Carbohydrate Chemistry
Genzyme Corporation
Lhiggins@genzyme.com
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From: pio@panther.cmb.umu.se [SMTP:pio@panther.cmb.umu.se]
Sent: Monday, August 16, 1999 9:30 AM
To: Recipients of ABRF List
Subject: Deglycosylation on PVDF
Dear all
We would like to in situ deglycosylation on a protein blotted to a
PVDF-membrane.
Has anyone experienced that? Can we find protocols for the reaction?
Thank you very much in advance,
Per-Ingvar Ohlsson
Per-Ingvar Ohlsson, Dr Med Sci, Department of Medical Biochemistry and
Biophysics, Umea University, S-907 87 Umea, Sweden
tel +46 90 786 6744, fax +46 90 136310, e-mail pio@panther.cmb.umu.se