Re: Cyanogen Bromide cleavage

I.L. Rodionov (rodionov@fibkh.serpukhov.su)
Wed, 18 Aug 1999 14:02:23 +0400

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Henry,
Below you will find 3 CNBr-related records which I have extracted from
Protean program (a part of DNASTAR package). Literature references are
not given.

1). CNBr with acids; RS:M^X,W^X; COM:Met is modified to Homoserine
Lactone and Trp is also modified and blocked on its C-Terminal.
2). CNBr, Cys methylated; RS:M^X,X^C; COM:Cys is modified to Serine. Met
is modified to Homoserine Lactone.
3). CNBr; RS:M^X; COM:Met is modified to Homoserine Lactone.Problem: Cys
could be oxydized (but it is reversible).

Regards,
Igor

------------------------------------------------
Igor L. Rodionov
Laboratory of Peptide Chemistry
Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
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Next message: Jacobs, Chris: "RE: Blank and partail blank runs on DNA Seq (377XL)"
Previous message: Bruce Stanley: "Re: Blank and partail blank runs on DNA Seq (377XL)"
Maybe in reply to: Henry Keutmann: "Cyanogen Bromide cleavage"