Re: Blank and partail blank runs on DNA Seq (377XL)

Robert Lyons (boblyons@umich.edu)
Wed, 18 Aug 1999 09:07:26 -0400

"Jacobs, Chris" wrote:
>
> sorry, we're in the same boat!
>
> I'll debuke their idea of the plates being dirty. Our 'blank' runs only
> occur on one of our instruments. The other instruments DO NOT have this
> problem.
> I've got to call them and see if we can get someone to check it out.

That was our situation as well, Chris. The problem plagued us for
almost 2 years on all machines until we bought the hot pots to heat
up wash water. It persisted on one machine until we started using
cerium oxide and then *immediately* disappeared. I don't think it's
coincidence. We had carefully eliminated every other possible cause.

On the other hand, everyone has to eliminate other causes themselves.
As another poster pointed out, the ddNTP ratio can cause a similar
effect, and bad spin columns could in theory do the same. After all,
it's not science, it's witchcraft. :)

Bob Lyons
U of Mich