My experience has been that destaining becomes helpful at lower
concentrations of loaded protein. When working at the range of 10pmol or
less, leaving the stain intact caused interference of the MALDI signal.
At high concentrations, however, there was no noticable affect. I use
the ferricyanide/thiosufate method, but I only keep the gel slices sitting
in the destaing sln as long as is absolutely neccessary. It works fine.
I hope this helps.
Angela Norbeck
University of Washington
On Thu, 19 Aug 1999, Dick Wicks wrote:
> Hello ABRFers:
>
> Has anyone looked at the ferricyanide/thiosulfate method to
> destain silver stained proteins from 2D gels before MALDI
> analysis? (Gharahdaghi et. al. Electrophoresis 20: 601, 1999). I
> would be especially interested in any side by side (destain vs. no
> destain) studies. Thanks.
>
>