silver destaining and ES

Roland_S_Annan@SBPHRD.com
Fri, 20 Aug 1999 09:40:25 -0400

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The few recent comments on the destaining of sliver stained bands only
discussed using MALDI for the analysis of the peptides. Has anyone tried
using nanoES or LC-MS/MS after destaining the silver stained spots. I'm
not sure why there would be any difference, but I was just wondering. For
those bands where we only get 1-2 peptide sequences, I'd be happy if the
destaining could get us a few more.

Roland S. Annan
Biological Mass Spectrometry
SmithKline Beecham Pharm

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