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Ok couple of suggestions:
1) Add the buffer after you have loaded odd samples then run for 5 mins and load evens (no buffer = no electrophoresis so of course there is going to be no separation between them).
2) Use a molecular weight ladder in every 5th lane. This means that you can still use an 8 channel loader to speed up the process and you only loose about 20 lanes from the 96 on the gel. Its a throughput versus hands on time equation really, the neural net tracker sucks so it takes more time to get the gel correct and to get the highest number of lanes on the gel you need it so load 77 samples and ignore the molecular weight markers.
3) Buy molecular weight markers from Microzone (www.microzone.co.uk) as they are half the cost of the ABI ones and work just as well
The extra dye marker that he is talking about, is a product that PE makes to make the tracking easier in the long run, however it will require a machine upgrade to get the virtual filter in there.
Let me know what you think.
David Cain
Comdisco U.K.
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I hope this helps!
Let me know if I can be of further assistance.
Best regards,
Richard
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Richard Tula
rptula@comdisco.com
Comdisco Laboratory & Scientific Group
50 Shawmut Road
Canton, MA 02021
800-545-0540 (Toll Free)
781-575-7104 (Direct)
781-575-0084 (Fax)
www.comdisco.com
>>> <Rdejackson@AOL.com> 08/17/99 05:45PM >>>
I work in a DNA sequencing core facility and we have been running a 377
with the 96 lane upgrade. The data has been looking pretty good since we
switched to the water loading protocol. The problem I am having is
distinguishing between lanes, there is no offset between the odd and even
lanes once you use the water protocol. I heard that there is a company that
makes a marker that can be added to the loading dye, perhaps in every other
lane, to help discriminate between lanes, does anyone know the companys name
or have any other suggestions?